The histology and micro-imaging core (HMC) facility at MWRI provides investigators with high quality, rapid tissue processing and imaging services. The core is designed with a “tissue to image” approach that allows investigators to request services, drop off samples and receive interpretation/images/image analyses of their processed samples according to their needs. The facility is directed by Carlos A. Castro, MD, DMD, an expert in perinatal and gynecological pathology with unique skills in fine and detailed dissections of small embryos as well as other surgical tissues. The HMC, which occupies over 730 ft2 of laboratory space on the 7th floor of MWRI, houses several integral pieces of equipment including a Leica CM1850UV cryostat for frozen tissue, a Thermo embedding station, Thermo, Microm and a Leica microtomes, a complete grossing station, and a manual staining station capable of performing routine, special, and in situ staining procedures, a Leica BondMax Immunostaining System for immunohistochemistry , and a Zeiss PALM MicroBeam Laser Capture Microdissection System. The core operates a routine Sakura Tissue-Tek VIP conventional tissue processor as well as a Milestone RHS microwave tissue processor, which has several advantages over conventional alcohol-based processing, including faster processing times and ease of use. In combination with special alcohol-based fixatives, the microwave-processed tissue allows the investigator to retrieve macromolecules (DNA, RNA, protein) from the paraffin block at similar quality and quantity compared to bulk flash frozen tissue. The HMC also houses a full-service micro-imaging and analysis suite that features a Nikon 90i, fully motorized upright microscope for capturing high quality and high-resolution bright field, DIC, and fluorescence images. The suite includes a Nikon A1 advanced confocal system with resonant scanner for live-imaging and spectral detection, allowing detections of up to 32 color channel with Volocity 3D/4D imaging and deconvolution software, a Leica DMIRE2 laser confocal microscope, and a Nikon Eclipse TE2000-E spinning disk confocal microscope. All three confocal microscopes are equipped for live cell imaging. A separate robust computer workstation that includes Photoshop, MetaMorph and Nikon Elements analysis software is available for more extensive post acquisition image analyses.

 

Histology Core Services and Fees

Services offered:

  1. Process fresh and fixed tissue specimens (grossing, tissue processing, embedding,
    sectioning).
  2. Microwave tissue processing (retrieve quality RNA/DNA from specially fixed paraffin-embedded blocks) and conventional alcohol-based tissue processing.
  3. Frozen sections (performed by investigator, tutorials by core).
  4. Routine, special, and immunohistochemical stains, including antibody work-up.
  5. Laser capture microdissection.
  6. Imaging (brightfield, epifluorescence, DIC; done in conjunction with core staff) and image analysis on the Nikon 90i fully motorized imaging platform using Nikon Elements AR software for acquisition and analysis (measuring objects/region of interest, quantifying IHC staining or other staining etc.).
  7. Training Sessions: Training sessions are available for the cryostat, LCM and Scope as well as consultation for special requests/projects through Dr. Castro.
  8. Dr. Castro and Mrs. Luketich are available for special consultation and interpretation of tissue based specimens.

 

Fee structure:

Contact the Core personnel for current pricing fee.

 

Checklist for users:

  1. Complete histology request form, or at the Core.
  2. Drop request form in the lab or e-mail completed histology request form to ccastro@mwri.magee.edu or lukeas@mail.magee.edu
  3. Drop off tissue and request in lab.

 

Scheduling Time for Histology Research and Micro-Imaging Research Core:

  • Cryostat time can be scheduled in Outlook at “MWR Histology Core Leica Cryostat”
    Service Account.
  • Microscope time can be scheduled in Outlook at “mwrnikon90i” Service Account.
  • LCM time can be scheduled in Outlook at “mwrlasercapture” Service Account.

 

Reservations requirements:

  • Reservation entries must include full name, lab #, extension, and the grant number to
    be charged.
  • Reservations must be limited to no more than 4 hours at one time.
  • Cancellations will be honored 4 hours prior to the start of your reservation. Users charged fee for a 4-hour time block if cancellation is not made on time.

 


 

New to the Histology Core? Not sure how this technology can help your research?

The Histology Core provides histology research and micro imaging research investigators with the highest quality tissue processing from fresh tissue to digital image and image analysis.

 

Links to protocols and related websites: