- Home
- Our Investigators
- Kyle Orwig, PhD
Kyle Orwig, PhD
Professor, Department of Obstetrics, Gynecology & Reproductive Sciences, University of Pittsburgh School of Medicine
Sometimes we talk about ‘how many people in the world are really impacted by infertility.’ For me, the only person that matters is the one that walks in the door today and desperately wants to have a biological child.”
Kyle Orwig, PhD
Research in Brief
Research in the Orwig laboratory (orwiglab.org) focuses on stem cells, germ lineage development, fertility and infertility. Our progress investigating reproductive function in fertile individuals provides a basis for understanding the mechanisms of infertility caused by disease, medical treatments, genetic defects or aging. Infertility impacts one in seven couples in the United States and can have a devastating impact on relationships and emotional well-being. The Orwig lab is ideally located in Magee-Womens Research Institute and UPMC Magee-Womens Hospital and is committed to translating lab bench discoveries to the clinic for diagnosis, prevention and treatment of infertility.
Current Projects
The Genome Editing, Transgenic and Virus Core Facility of Magee-Womens Research Institute
The Genome Editing, Transgenic and Virus Core Facility of Magee-Womens Research Institute provides state-of-the-art services for generating transgenic mice/rats and knockout mice. The facility also produces replication defective lentiviral vectors for delivering recombinant transgenes and performs teratoma and chimera analyses to evaluate stem cell developmental potential.
The Fertility Preservation Program of Pittsburgh
The Fertility Preservation Program of Pittsburgh educates patients and their physicians about the reproductive consequences of chemotherapy and radiation treatments for cancer or other conditions while developing pioneering reproductive technologies to preserve fertility. The program rapidly translates these state-of-the-art technologies to the clinic, and trains the next generation of clinicians and researchers in the rapidly evolving discipline of fertility preservation.
Selected Publications
- Hardy JJ, Wyrwoll MJ, Mcfadden W, Malcher A, Rotte N, Pollock NC, Munyoki S, Veroli MV, Houston BJ, Xavier MJ, Kasak L, Punab M, Laan M, Kliesch S, Schlegel P, Jaffe T, Hwang K, Vukina J, Brieño-Enríquez MA, Orwig K, Yanowitz J, Buszczak M, Veltman JA, Oud M, Nagirnaja L, Olszewska M, O’Bryan MK, Conrad DF, Kurpisz M, Tüttelmann F, Yatsenko AN. Variants in GCNA, X-linked germ-cell genome integrity gene, identified in men with primary spermatogenic failure. GEMINI Consortium. Hum Genet. 2021 May 7. doi: 10.1007/s00439-021-02287-y. [PUBMED]
- Voigt AL, Kondro DA, Powell D, Valli-Pulaski H, Ungrin M, Stukenborg JB, Klein C, Lewis IA, Orwig KE, Dobrinski I. Unique metabolic phenotype and its transition during maturation of juvenile male germ cells. FASEB J. 2021 May;35(5):e21513. doi: 10.1096/fj.202002799R. [PUBMED]
- Hinkle K, Orwig KE, Valli-Pulaski H, Taylor S, van Leeuwen K, Carpentieri D, Walsh A. Cryopreservation of Ovarian Tissue for Pediatric Fertility. Biopreserv Biobank. 2021 Apr 12. doi: 10.1089/bio.2020.0124. [PUBMED]
- Parker, N, Laychur A, Sukhwani M, Orwig KE, Oatley JM, Zhang C, Rutaganaria FU, Shokat K, Wright WW. Spermatogonial stem cell numbers are reduced by transient inhibition of GDNF signaling but restored by self-renewing replication when signaling resumes. Stem Cell Reports. 2021 March. 16(3): 597–609. doi: 10.1016/j.stemcr.2021.01.015. [PUBMED]
- Shetty G, Mitchell JM, Lam TNA, Phan TT, Zhang J, Tailor RC, Peters KA, Penedo MC, Hanna CB, Clark AT, Orwig KE, Meistrich ML. Restoration of Functional Sperm Production in Irradiated Pubertal Rhesus Monkeys by Spermatogonial Stem Cell Transplantation. Andrology 2020; doi: 10.1111/andr.12807. [PUBMED]
- Yersal N, Köse S, Horzum U, Özkavukcu S, Orwig KE, Korkusuz P. Leptin promotes proliferation of neonatal mouse stem/progenitor spermatogonia. J Assist Reprod Genet. 2020 Aug 25;. doi: 10.1007/s10815-020-01929-w. [PUBMED]
- Tan K, Song HW, Thompson M, Munyoki SK, Sukhwani M, Hsieh TC, Orwig KE, Wilkinson MF. Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro. PNAS. 2020 Jul 13;202000362. doi: 10.1073/pnas.2000362117. [PUBMED]
- Goossens E, Jahnukainen K, Mitchell RT, van Pelt A, Pennings G, Rives N, Poels J, Wyns C, Lane S, Rodriguez-Wallberg KA, Rives A, Valli-Pulaski H, Steimer S, Kliesch S, Braye A, Andres MM, Medrano J, Ramos L, Kristensen SG, Andersen CY, Bjarnason R, Orwig KE, Neuhaus N, Stukenborg JB. Fertility Preservation in Boys: Recent Developments and New Insights. Hum Reprod Open. 2020. doi: 10.1093/hropen/hoaa016. eCollection 2020. [PUBMED]
- Meacham LR, Burns K, Orwig KE, Levine J. Standardizing risk assessment for treatment-related gonadal insufficiency and infertility in childhood adolescent and young adult cancer: The Pediatric Initiative Network risk stratification system. J Adolesc Young Adult Oncol. 2020 doi: 10.1089/jayao.2020.0012. [PUBMED]
- Nahata L, Woodruff TK, Quinn GP, Meacham LR, Chen D, Appiah LC, Finlayson C, Orwig KE, Laronda MM, Rowell EE, Anazodo A, Frias O, Rios JS, Whiteside S, Gomez-Lobo V, Dwiggins M, Childress KJ, Hoefgen HR, Levine JM, Jayasinghe Y, Moravek M. Ovarian tissue cryopreservation as standard of care: what does this mean for pediatric populations? J Assist Reprod Genet. 2020; doi: 10.1007/s10815-020-01794-7. [PUBMED]
- Shami AN, Zheng X, Munyoki SK, Ma Q, Manske GL, Green GD, Sukhwani M, Orwig KE, Li JL*, Hammoud SS. Single-cell RNA sequencing of human, macaque, and mouse testes uncovers conserved and divergent features of mammalian spermatogenesis. Dev Cell 2020, 54(4):529-547.e12. doi: 10.1016/j.devcel.2020.05.010. [PUBMED]
- Shetty G, Mitchell JM, Meyer JM, Wu Z, Lam TNA, Phan TT, Zhang J, Hill L, Tailor RC, Peters KA, Penedo MC, Hanna C, Orwig KE, Meistrich ML. Restoration of Functional Sperm Production in Irradiated Pubertal Rhesus Monkeys by Spermatogonial Stem Cell Transplantation. .Andrology 2020; doi: 10.1111/andr.12807. [PUBMED]
- Morimoto H, Kanatsu-Shinohara M, Orwig KE, Shinohara T. Expression and functional analyses of EPHA2 in mouse spermatogonial stem cells. Biol Reprod. 2019 Aug 12. pii: ioz156. doi: 10.1093/biolre/ioz156. [PUBMED]
- Barnard EP, Dhar CP, Rothenberg SS, Menke MN, Witchel SF, Montano GT, Orwig KE, Valli-Pulaski H. Fertility Preservation Outcomes in Adolescent and Young Adult Feminizing Transgender Patients. Pediatrics. 2019 Sep;144(3). pii: e20183943. doi: 10.1542/peds.2018-3943. [PUBMED]
- Valli-Pulaski H, Peters KA, Gassei K, Steimer SR, Sukhwani M, Hermann BP, Dwomor L, David S, Fayomi AP, Munyoki SK, Chu T, Chaudhry R, Cannon GM, Fox PJ, Jaffe TM, Sanfilippo JS, Menke MN, Lunenfeld E, Abofoul-Azab M, Sender LS, Messina J, Klimpel LM, Gosiengfiao Y, Rowell EE, Hsieh MH, Granberg CF, Reddy PP, Sandlow JI, Huleihel M, Orwig KE. Testicular tissue cryopreservation: 8 years of experience from a coordinated network of academic centers. Hum Reprod. 2019; May 21; doi: 10.1093/humrep/dez043. [PUBMED]
- Sakib S, Uchida A, Valenzuela-Leon P, Yu Y, Valli-Pulaski H, Orwig K, Ungrin M, Dobrinski I. Formation of organotypic testicular organoids in microwell culture. Biol Reprod. 2019; March 29; doi: 10.1093/biolre/ioz053. [PUBMED]
- Fayomi A, Peters K, Sukhwani M, Valli-Pulaski H, Shetty G, Meistrich M, Houser L, Robertson N, Roberts V, Ramsey C, Hanna C, Hennebold J, Dobrinski I, Orwig KE. Autologous Grafting of Cryopreserved Prepubertal Rhesus Testis Produces Sperm and Offspring. Science. 2019; March 22; 363(6433): 1314-1319. doi: 10.1126/science.aav2914. [PUBMED]