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MWRI’s Genome Editing, Transgenic and Virus (GETV) Core Facility provides leadership, technical expertise, advice, and training for investigators both within and outside the University of Pittsburgh community. The core is comprised of three functional sections:
- Molecular cloning (plasmid, virus, CRISPR/Cas9)
- Viral vectors (lentivirus, adenovirus, adeno-associated virus)
- Animal services (transgenic, knockout, cryopreservation, re-derivation and more)
- Assistance for generating recombinant plasmids
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work
- Mouse and rat transgenesis, including transgenic and knockout/knockdown techniques using pronuclear injection, ES cell injection/chimera and CRISPR/Cas9-mediated genome editing
- Production of lentivirus, adenovirus and adeno-associated virus vectors.
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines; cryopreserve or re-derive of valuable transgenic lines (sperm or embryos).
- GETV services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh.