MWRI’s Genome Editing, Transgenic, and Virus (GETV) Core Facility provides leadership, technical expertise, advice, and training for investigators both within and outside the University of Pittsburgh community. The core is comprised of three functional sections:

• Molecular cloning (plasmid, virus, CRISPR/Cas9)
• Viral vectors (lentivirus, adenovirus, adeno-associated virus)
• Animal services (transgenic, knockout, cryopreservation, re-derivation, and more)

Core Services

Services offered:

• Assistance for generating recombinant plasmids
• Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work
• Mouse and rat transgenesis, including transgenic and knockout/knockdown techniques using pronuclear injection, ES cell injection/chimera, and CRISPR/Cas9-mediated genome editing
• Production of lentivirus, adenovirus, and adeno-associated virus vectors
• Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines, cryopreserve or re-derive of valuable transgenic lines (sperm or embryos)
• GETV services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh

Fee Structure:

Contact core personnel for current pricing fee.

Location

Genome Editing, Transgenic, and Virus (GETV) Core
Magee-Womens Research Institute
204 Craft Avenue, Lab B730
Pittsburgh, PA 15213

Contact

Core Director

Kyle Orwig
E: orwigke@upmc.edu

Core Manager

Yi Sheng
E: ysheng@mwri.magee.edu

Administrator

Jennifer Orwig
E: jshuttleworth@mwri.magee.edu
P: 412-641-2415